Skip to content.

TUD

search  |  internal  |  deutsch
Personal tools
TU Dresden » Faculty of Mechanical Science and Engineering » Institute for Materials Science » Chair of Materials Science and Nanotechnology

For a complete list, check out Google Scholar.

prev/next pubs | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002 | 2001 | 2000 | 1999 | 1998 | 1997 | 1996

[back]


145. 


Stromal cell-derived factor-1α-directed chemoattraction of transiently CXCR4-overexpressing bone marrow stromal cells into functionalized three-dimensional biomimetic scaffolds



S. Thieme, M. Ryser, M. Gentsch, K. Navratiel, S. Brenner, M. Stiehler, J. Rölfing, M. Gelinsky, A. Rösen-Wolff

Tissue Engineering: Part C 15, 687 (2009)

Three-dimensional (3D) bone substitute material should not only serve as scaffold in large bone defects but also attract mesenchymal stem cells, a subset of bone marrow stromal cells (BMSCs) that are able to form new bone tissue. An additional crucial step is to attract BMSCs from the surface into deeper structures of 3D porous bone substitute scaffolds. Here we show that transient overexpression of CXCR4 in human BMSCs induced by mRNA transfection enhances stromal cell-derived factor-1α (SDF-1α)–directed chemotactic capacity to invade internal compartments of porous 3D bone substitute scaffolds in vitroand in vivo. In vitronative BMCSs invaded up to 500 μm into SDF-1α–releasing 3D scaffolds, whereas CXCR4-overexpressing BMSCs invaded up to 800 μm within 5 days. In addition, 60% downregulation of endogenous SDF-1 transcription in BMSCs by endoribonuclease-prepared siRNA before CXCR4 mRNA transfection enhanced SDF-1α–directed migration of human BMSCs by 50%. Implantation of SDF-1α–releasing scaffolds seeded with transiently CXCR4-overexpressing BMSCs resulted in an increase of invasion into internal compartments of the scaffolds in a mouse model. In vivonative BMCS invaded up to 250 μm into SDF-1α–releasing 3D scaffolds, whereas CXCR4-overexpressing BMSC invaded up to 500 μm within 5 days. Thus, the SDF-1α/CXCR4 chemoattraction system can be used to efficiently recruit BMSCs into SDF-1α–releasing 3D scaffolds in vitroand in vivo.



doi absolute link10.1089/ten.tec.2008.0556
online abstract at the publisher site
publisher site
export BibTeX citation (txt file)
export EndNote citation (ris file)


prev/next 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002 | 2001 | 2000 | 1999 | 1998 | 1997 | 1996

[back]



















































last modified: 2018.10.24 Mi
author: webadmin