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424. 


Chemiresistive biosensors based on carbon nanotubes for label-free detection of DNA sequences derived from avian influenza virus H5N1



Yangxi Fu, Victor Romay, Ye Liu, Bergoi Ibarlucea, Larysa Baraban, Vyacheslav Khavrus, Steffen Oswald, Alicja Bachmatiuk, Imad Ibrahim, Mark R¸mmeli, Thomas Gemming, Viktor Bezugly, Gianaurelio Cuniberti

Sensors and Actuators B: Chemical 691-699 (2017)

We developed chemiresistor-type biosensors based on carbon nanotubes for highly efficient and fast detection of avian influenza virus (AIV) subtype H5N1 DNA sequences. Semiconducting single-walled carbon nanotubes (sc-SWCNTs) or nitrogen-doped multi-walled carbon nanotubes (N-MWCNTs) were used as two alternative active sensing elements, and their sensitivity to different concentrations of DNA target were compared. In these sensors long nanotubes (>5†µm) were placed between interdigitated metal electrodes so that individual nanotubes connect the electrodes. The nanotubes were functionalized with DNA probe sequences non-covalently attached to the sidewalls. Such functionalized-nanotube sensors could reliably detect complementary DNA target sequences of the AIV H5N1 with concentration ranging from 2†pM to 2†nM in 15†min and at room temperature. Our nanotube-based biosensors are small, flexible, disposable and easy-to-fabricate that makes them promising for point-of-care applications and clinical diagnostics.



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doi absolute linkdoi.org/10.1016/j.snb.2017.04.080
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Prof. Dr. Gianaurelio Cuniberti
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