Human thrombin detection platform using aptamers on a silicon nanowire field-effect transistor
L. Römhildt, F. Zörgiebel, B. Ibarlucea, M. Vahdatzadeh, L. Baraban, G. Cuniberti, S. Pregl, W. M. Weber, T. Mikolajick, and J. Opitz
27th International Symposium on Power and Timing Modeling, Optimization and Simulation (IEEE PATMOS) (2017)
We present a silicon nanowire-based field-effect transistor biosensor with Schottky barriers for highly specific and sensitive human α-thrombin detection. The active sensor area is decorated with thrombin-binding aptamers as receptor molecules. Each sensor chip is integrated into a microfluidic device for flow-through measurements. Instantaneous detection is provided by real-time monitoring of FET transfer curves. With this approach, thrombin concentrations between 200 pM and 200 nM are detected in a label-free, real-time manner, covering a wide dynamic range and enabling to distinguish between healthy and pathological levels. The development of simple, miniaturized devices for blood protein detection is highly interesting for medical diagnostics.