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TU Dresden » Faculty of Mechanical Science and Engineering » Institute for Materials Science » Chair of Materials Science and Nanotechnology



Thursday, 28 April 2011
(at 13:00 in room 115, Hallwachsstr. 3)
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Molecular-level assessment of disease-relevant mechanisms by atomic force microscopy

Adrian Keller

Interdisciplinary Nanoscience Center (iNANO)
Aarhus University
  Denmark  






Atomic force microscopy (AFM) is a versatile imaging technique with nanometer resolution that is ideally suited for investigating organic and inorganic nanostructures on solid surfaces. It has proven particularly useful in the study of biological macromolecules such as proteins or DNA where it may provide unique information on molecular structure, conformation and aggregation. We have thus applied AFM to study disease-relevant molecular aggregation and fragmentation mechanisms in vitro. The denaturation and aggregation of peptides and proteins into highly-ordered amyloid nanofibrils plays an important role in the development of so-called misfolding diseases such as Alzheimer's disease, Parkinson's disease, or prion disease. In the physiological environment, amyloid aggregation is affected by the presence of interfaces such as cell membranes. By using a novel type of model surface, we were able to investigate the influence of surface hydrophobicity on the surface-catalyzed aggregation of the islet amyloid polypeptide which is critical for the development of type 2 diabetes mellitus. In addition, we present a new AFM-based strategy for the assessment of DNA damage induced by low-energy electrons and ions which are generated as secondary particles in the interaction of biological matter with ionizing radiation, e.g. in radiation tumor therapy. Here, AFM provides the unique possibility to study the fragmentation of complex DNA structures at the single-molecule level.

Brief Bio:

Adrian Keller received his diploma in engineering physics from the University of Applied Sciences Coburg in 2005. In his PhD studies at the Forschungszentrum Dresden-Rossendorf (now Helmholtz-Zentrum Dresden-Rossendorf) he worked on ion-induced nanoscale pattern formation. In 2009, he received his doctoral degree from TU Dresden and was awarded a postdoc fellowship from the Alexander von Humboldt foundation for which he joined the scanning probe microscopy group of Prof. Flemming Besenbacher at the Interdisciplinary Nanoscience Center at Aarhus University. His current research focuses on biological model surfaces, protein aggregates, and DNA structures.



slides (pdf)

Invited by G. Cuniberti

Within the nanoSeminar

last modified: 2018.10.24 Mi
author: webadmin