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TU Dresden » Faculty of Mechanical Science and Engineering » Institute for Materials Science » Chair of Materials Science and Nanotechnology

Thursday, 16 May 2013
(at 13:00 in room HAL 115)
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Biophysical Characterization of Plasma Membrane Structure and Dynamics via a combined AFM/FCS Approach

Salvatore Chiantia

Humboldt-Universitä zu Berlin

The lateral organization of lipids and membrane receptors in the plasma membrane (PM) is connected to a large variety of biological processes including immune response, cell-cell communication, membrane trafficking and some bacterial and viral infections. A growing amount of data suggest that chemically selective assemblies of lipids and proteins can exist in cellular membranes and that they have a role in several biological contexts. The characterization of such domains in vivo has proved so far to be very challenging due to their small size and dynamic nature and therefore requires the design of innovative, versatile and effective experimental approaches. On the other hand, the improvement of existing membrane physical models is also of vital importance for the full understanding of cell membrane function through the biophysical characterization of lipid-protein interactions. In the first part of my presentation, I will describe a combination of Atomic force microscopy (AFM) and Fluorescence Correlation Spectroscopy (FCS) that proved particularly useful for the characterization of structural and dynamic properties of artificial model PM containing "raft" and ceramide domains. In the second part, I will describe novel and advanced asymmetric model bilayers (e.g. asymmetric giant unilamellar vesicles or GUVs) which mimic the compositional lipid asymmetry of cellular PM.

Brief Bio:

Salvo Chiantia studied Physics in the University of Palermo where he graduated in 2003. In 2008, he received his PhD in Physics under the supervision of Prof. Schwille in the TU-Dresden, working on a combination of AFM and single-molecule fluorescence for the study of lipid membranes. Between 2009 and 2012, he worked as a research scientist in the group of Prof. London (Stony Brook University, NY) where he developed a method for the production of asymmetric giant lipid vesicles (GUVs). At the present, he is holding a PostDoc position in the Department of Biology / Molekulare Biophysik at the HU-Berlin.

Announcement (pdf)

slides (pdf)

Invited by G. Cuniberti

Within the nanoSeminar

last modified: 2021.10.02 Sat
author: webadmin