Human thrombin detection platform using aptamers on a silicon nanowire field-effect transistor
27th International Symposium on Power and Timing Modeling, Optimization and Simulation (IEEE PATMOS) , 0 (2017).
L. Römhildt, F. Zörgiebel, B. Ibarlucea, M. Vahdatzadeh, L. Baraban, G. Cuniberti, S. Pregl, W. M. Weber, T. Mikolajick, and J. Opitz.
Journal DOI: https://doi.org/10.1109/PATMOS.2017.8106958

We present a silicon nanowire-based field-effect transistor biosensor with Schottky barriers for highly specific and sensitive human α-thrombin detection. The active sensor area is decorated with thrombin-binding aptamers as receptor molecules. Each sensor chip is integrated into a microfluidic device for flow-through measurements. Instantaneous detection is provided by real-time monitoring of FET transfer curves. With this approach, thrombin concentrations between 200 pM and 200 nM are detected in a label-free, real-time manner, covering a wide dynamic range and enabling to distinguish between healthy and pathological levels. The development of simple, miniaturized devices for blood protein detection is highly interesting for medical diagnostics.

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©10.1109/PATMOS.2017.8106958
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Human thrombin detection platform using aptamers on a silicon nanowire field-effect transistor
27th International Symposium on Power and Timing Modeling, Optimization and Simulation (IEEE PATMOS) , 0 (2017).
L. Römhildt, F. Zörgiebel, B. Ibarlucea, M. Vahdatzadeh, L. Baraban, G. Cuniberti, S. Pregl, W. M. Weber, T. Mikolajick, and J. Opitz.
Journal DOI: https://doi.org/10.1109/PATMOS.2017.8106958

We present a silicon nanowire-based field-effect transistor biosensor with Schottky barriers for highly specific and sensitive human α-thrombin detection. The active sensor area is decorated with thrombin-binding aptamers as receptor molecules. Each sensor chip is integrated into a microfluidic device for flow-through measurements. Instantaneous detection is provided by real-time monitoring of FET transfer curves. With this approach, thrombin concentrations between 200 pM and 200 nM are detected in a label-free, real-time manner, covering a wide dynamic range and enabling to distinguish between healthy and pathological levels. The development of simple, miniaturized devices for blood protein detection is highly interesting for medical diagnostics.

Cover
©10.1109/PATMOS.2017.8106958
Share


Involved Scientists