Specific biomarkers detected in body fluids and tissues are crucial elements for disease diagnosis, prediction of its progression, and evaluation of personalised treatment effectiveness. Although the detection of a single specific biomarker is sufficient, the simultaneous detection of multiple disease-associated biomarkers (cancer biomarkers, inflammatory proteins, etc.) significantly improves the diagnostic power of biomarkers in terms of specificity and sensitivity from the Receiver operating characteristic curve (ROC).
Electrochemical biosensors meet the criteria of Point-of-Care (POCT) devices and represent the alternative to commonly used instrumentally and time-consuming laborious diagnostic methods (ELISA, immunohistochemistry, PCR). Among electrochemical biosensors, immunosensors, based on the principle of highly specific affinity interactions between antigens and two corresponding antibodies identical to the ELISA method, are of interest in clinical diagnosis. Increasingly, the common enzyme-based immunosensors are being replaced by nanomaterials-based sensors to overcome the shortcomings of enzymes. From electroactive nanoparticles, gold nanoparticles (AuNPs), silver nanoparticles (AgNPs), quantum dots (QDs), carbon nanotubes (CNTs), graphene, and carbon quantum dots (CQDs) are the most commonly used. Moreover, these nanomaterials enable the simultaneous detection of multiple biomarkers since they provide separate, non-interfering and mutually unaffected electrochemical signals.
The talk will be focused on the development of electrochemical magneto-immunoassay-based sensors for simultaneous stripping voltammetric detection of ovarian cancer biomarkers, namely proteins HE4, CA125, AFP, and pro-inflammatory biomarkers PTX3 and CALR, as potential predictive markers of preterm labour.
Zuzana Bilkova graduated in Molecular Biology and Genetics from Charles University, Prague, Czech Republic. In 2000 she received the Ph.D. in Analytical chemistry from Faculty of Chemical Technology, University of Pardubice. In 1998 she joined the Department of Biological and Biochemical Sciences as a lecturer, and in 2005 she became a head of department. In 2014, she was promoted to Professor of Clinical Immunology. Her current research interests include bioanalytical methods based on molecular recognition and use of modern (nano)materials as solid phase, often combined with microfluidic systems or biosensors. Her scientific expertise: immunology, immunochemistry, bioaffinity chromatography, and structural analysis of clinically relevant biomarkers. She is a member of many scientific and advisory boards. Up to the present day, she has published more than 115 papers in scientific journals (H-Index 26, WOS 2024), and she gave more than 30 invited lectures at symposiums and conferences.
Lucie Korecka graduated at the Faculty of Chemical Technology, University of Pardubice, Czech Republic in Analysis of biological materials in 2001. In 2005 she received the Ph.D. in Analytical chemistry at the same faculty. In 2004 she received RNDr. degree and in 2021 she completed her habilitation procedure in Biochemistry, both at Palacký University in Olomouc. Since 2006 she is lecturer at the Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice. Her scientific expertise is mainly focused on immunochemistry, biochemistry, enzymology, immobilization techniques, biofunctionalization of micro and nanomaterials and electrochemical immunosensors. She is experienced also in synthesis of biocompatible hyaluronic acid nanoparticles and they in-synthetic and post-synthetic modification with potential for drug delivery. She is (co-)author of 31 original papers in peer reviewed impacted journals with 428 overall citations (eithout self-citations) according to WOS, co-investigator of 1 Czech patent application; H index 13.
Specific biomarkers detected in body fluids and tissues are crucial elements for disease diagnosis, prediction of its progression, and evaluation of personalised treatment effectiveness. Although the detection of a single specific biomarker is sufficient, the simultaneous detection of multiple disease-associated biomarkers (cancer biomarkers, inflammatory proteins, etc.) significantly improves the diagnostic power of biomarkers in terms of specificity and sensitivity from the Receiver operating characteristic curve (ROC).
Electrochemical biosensors meet the criteria of Point-of-Care (POCT) devices and represent the alternative to commonly used instrumentally and time-consuming laborious diagnostic methods (ELISA, immunohistochemistry, PCR). Among electrochemical biosensors, immunosensors, based on the principle of highly specific affinity interactions between antigens and two corresponding antibodies identical to the ELISA method, are of interest in clinical diagnosis. Increasingly, the common enzyme-based immunosensors are being replaced by nanomaterials-based sensors to overcome the shortcomings of enzymes. From electroactive nanoparticles, gold nanoparticles (AuNPs), silver nanoparticles (AgNPs), quantum dots (QDs), carbon nanotubes (CNTs), graphene, and carbon quantum dots (CQDs) are the most commonly used. Moreover, these nanomaterials enable the simultaneous detection of multiple biomarkers since they provide separate, non-interfering and mutually unaffected electrochemical signals.
The talk will be focused on the development of electrochemical magneto-immunoassay-based sensors for simultaneous stripping voltammetric detection of ovarian cancer biomarkers, namely proteins HE4, CA125, AFP, and pro-inflammatory biomarkers PTX3 and CALR, as potential predictive markers of preterm labour.
Zuzana Bilkova graduated in Molecular Biology and Genetics from Charles University, Prague, Czech Republic. In 2000 she received the Ph.D. in Analytical chemistry from Faculty of Chemical Technology, University of Pardubice. In 1998 she joined the Department of Biological and Biochemical Sciences as a lecturer, and in 2005 she became a head of department. In 2014, she was promoted to Professor of Clinical Immunology. Her current research interests include bioanalytical methods based on molecular recognition and use of modern (nano)materials as solid phase, often combined with microfluidic systems or biosensors. Her scientific expertise: immunology, immunochemistry, bioaffinity chromatography, and structural analysis of clinically relevant biomarkers. She is a member of many scientific and advisory boards. Up to the present day, she has published more than 115 papers in scientific journals (H-Index 26, WOS 2024), and she gave more than 30 invited lectures at symposiums and conferences.
Lucie Korecka graduated at the Faculty of Chemical Technology, University of Pardubice, Czech Republic in Analysis of biological materials in 2001. In 2005 she received the Ph.D. in Analytical chemistry at the same faculty. In 2004 she received RNDr. degree and in 2021 she completed her habilitation procedure in Biochemistry, both at Palacký University in Olomouc. Since 2006 she is lecturer at the Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice. Her scientific expertise is mainly focused on immunochemistry, biochemistry, enzymology, immobilization techniques, biofunctionalization of micro and nanomaterials and electrochemical immunosensors. She is experienced also in synthesis of biocompatible hyaluronic acid nanoparticles and they in-synthetic and post-synthetic modification with potential for drug delivery. She is (co-)author of 31 original papers in peer reviewed impacted journals with 428 overall citations (eithout self-citations) according to WOS, co-investigator of 1 Czech patent application; H index 13.
